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ABOUT US

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Proteomics Core

The mission of the Proteomics Core is to analyze the structure, function, and interactions of proteins and to promote awareness, collaboration, and expand proteomics applications and research. The Core is housed on the 3rd floor of the CSRB-LSUHSC.

Services include 2D gel electrophoresis analysis, fluorescent tags and stains, MS-HPLC, protein ID in quantization and biomarker discovery.

Equipment
BioRad 2-D gel electrophoresis system, BioRad GS800 densitometer, ProteomeWorks Spot Cutter with PDQuest software for gel imaging, spot picking, analysis, and a Perkin-Elmer Multi-Probe II sample handling workstation. Equipment accommodating fluorescent tags and special purposed stains (phosphorylation, glycosylation): an Amersham IPGPhor and Ettan 2-D gel electrophoresis system, a Typhoon imaging system, and an Ettan handling workstation. A Dionex nano- to micro-flow LC multiple dimensional high performance liquid chromatography system can run parallel ion exchange and reversed phase columns for multiple dimensional LC separation to realize off-line mass spectrometry sample preparation. Mass Spectrometry equipment includes: ABI 4700 Proteomics Analyzer MALDI TOF-TOF mass spectrometer with Global Proteome Server Explorer workstation (with Mascot), designed for fast protein identification. Interfacing with off-line Dionex LC allows more efficient in quantization and biomarker discovery. ABI classic Voyager DE MALDI -TOF mass spectrometers are used as easy to operate walk-on instruments available on a charge per time basis. These instruments are excellent for MW determination of small proteins/peptides and other biological samples such as oligosaccharides. Thermo-Fisher LTQ XL Ion Trap Mass Spectrometer with Eksigent 2D nanoLC possesses great sensitivity and can provide LC-based total proteome approach, peptide sequencing, and detecting modifications, such as phosphorylation and acetylation. For data analysis there is an IBM 10-node parallel processor server will run 5-node Sequest and 5-node Mascot software to analyze data from LTQ and 4700, for protein ID search, database blast, and modification determination.

The director of the Proteomics Core is Chau-Wen Chou PhD.

 

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