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Imaging Core

The mission of the Imaging Core is to provide histology, microcopy and imaging services to support and advance research activities. The Core is located in LSUHSC Gene Therapy Program (CSRB 5th floor).

Services include expert advice on experimental design and interpretation of results.

Equipment

Leica CM3050S Cryostat with CryoJane Module, a Shandon Hypercenter XP Paraffin Processor, a Shandon Histocentre Paraffin Embedding Station, a Shandon Finesse ParaffinMicrotome, a Leica DMRXA deconvolution microscopy system, a BioRad Radiance 2100 Laser Scanning Confocal System, a Nikon E600 brightfield/epifluorescence microscopy system, and a Leica Mz75 brightfield/epifluorescence stereomicroscopy system.

A P.A.L.M. Laser Microdissection System (capable of dissecting minute fragments of target tissue from sections for detailed histological, immunological or genomic analyses). This offers significant advantages to researchers for gene-based and phenotypic analyses of minute regions of tissue (as few as 10 cells) in studies of host/pathogen interaction.

A Xenogen IVIS® SPECTRUM imaging system allows real-time imaging to non-invasively monitor and record cellular and genetic activity within a living organism with attendant advantages of high throughput (of mice) and high-sensitivity in vivo imaging of both fluorescence and bioluminescence with resolution (at 3.9cm field) to 20 microns.

This equipment will facilitate in vivo localization and tracking of labeled cellular and genetic material within an organism in studies of disease pathogenesis and host response.

An Olympus Fluoview FV1000 multiphoton laser scanning microscope equipped with a Mai Tai Titanium: Sapphire laser to allow fluorescence imaging deep within specimens. Utilizing nanosecond-pulsed infrared illumination, the Fluoview MPE (multiphoton excitation) is able to image up to 1mm into a specimen thanks to the scatter-free penetration of infrared light and long working distance objectives.

The system’s software modules are easy to use and include flexibility in scanning modes and applications including time-course, FRET, FRAP, photo-stimulation, 3D rendering, spectral un-mixing (up to 2nm), co-localization, and multi-point observation.

The Imaging Core is managed by Luis Marrero.

Click here for Imaging Core Slide Show.